Western blot was performed using anti-ENO1 Monoclonal Antibody (Product # MA5-32756) on whole cell extracts (30 µg lysate) of THP-1 (Lane1), A549 (Lane 2), U-87 MG (Lane 3), Mouse heart (Lane 4), Mouse skeletal muscle (Lane 5) and Rat skeletal muscle (Lane 6) and 47 kDa band corresponding to ENO1 protein was observed across all the samples tested except in Mouse Heart. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).